The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. A was added to eppendorf tubes to a final volume of 20. All prices and stock availability are for informational purposes only. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of. Thermo scientific generuler 100 bp dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 100 bp to 1,000 bp on agarose or polyacrylamide gels. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Thermo scientific ogeneruler 1 kb dna ladder, readyto. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. Plasmid dna digested in 5 min with fastdigest xhoi. Researchcart360 does not make any representations or warranties as to the accuracy or timeliness of such information, which may be subject to change without notice. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. Thermo scientific fermentas fastdigest restriction enzymes. Thermo scientific generuler 1kb dna ladder, readytouse, is designed for sizing and approximate quantification of wide range double.
Ladder profile and sizes of thermoscientific generuler 100 bp dna ladder plus sm0243, sm0323. Readytoload 1 kb dna ladder ranging from 500 to 10,000 bp. Generuler 100 bp dna ladder thermo fisher scientific. The blue dye migrates with 35 kb dna fragments in a 1% agarose gel and has an excitation peak of 424 nm. Readytoload 1 kb dna ladder ranging from 500 to 10,000. William smith general phone 2755731 molecular biology sales specialist.
Generuler 1kb dna ladder, readytouse application of the substance the preparation. How can i prepare working solution with 1 kb dna ladder. Product information thermo scientific generuler 1 kb dna. The invitrogen 100 bp dna ladder is designed for sizing and. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes. This marker is ideal for the size determination of digested dna.
The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Generuler 1 kb plus dna ladder 75 to 20,000 bp is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Mix gently, but throughly, to ensure that the solution is homogeneous. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. Wide range of dna ladders and molecular weight markers from bioneer. Ladder profile and sizes of fermentas 1kb dna ladder sm0311. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. Thermo scientific generuler 1 kb dna ladder, readytouse. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels.
The ladder is composed of fourteen chromatographypurified. Generuler 1 kb dna ladder thermo fisher scientific. The ladder is composed of fifteen chromatographypurified individual dna fragments in base pairs. The ladder is a mixture of chromatographypurified individual dna fragments. Hyperladder 1kb with 1% wv agarose gel, hyperladder 50bp with 1.
The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Generuler 1 kb plus dna ladder can be labeled radioactively with t4 polynucleotide kinase. Load on sdspolyacrylamide gel for minigels with a thickness of 0. Follow the recommendations for loading described in the certificate of analysis of the dna laddersmarkers 0. Thermo scientific generuler 1 kb plus dna ladder fisher. Description thermo scientific generuler 1 kb dna ladder is. Ii total rna were analysed on a 1 % formaldehyde agarose gel. U of rochester biological supply ctr g8633 stock manager. Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp.
Thermo scientific fermentas fastdigest enzymes are an advanced line of restriction enzymes for rapid dna. The dna ladder consists of 10 dna fragments and is provided with 6x tritrack dna loading dye. Thermo scientific generuler 1 kb dna ladder perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this mixture of chromatographypurified individual dna fragments. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Description the generuler 1kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded. Thermo scientific generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Sigmaaldrich offers a number of directload 1 kb dna ladder products.
The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. Please have a look into the related application note for more information about ladder migration in different gel concentrations. Thermo scientific ogeneruler 1 kb dna ladder, readytouse 25010,000 bp perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this 1 kb dna ladder, premixed with blue and yellow tracking dyes. Product information thermo scientific generuler 1 kb. Generuler 1 kb dna ladder, readytouse catalog number sm03, sm0314 pub. Contents amount storage sm03 generuler 1 kb dna ladder, readytouse 250 5 x 50 g for 500 applications, 0. Thermo scientific generuler 1 kb dna ladder, readytouse 11571595 print thermo scientific generuler 1 kb dna ladder, readytouse perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Comes supplied with 1 vial of gel loading dye, purple 6x, no sds. Neb also offers a quickload version of this ladder with purple dye. Page 14 material safety data sheet prepared in accordance with eu directive 91155eec printed. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. In addition to these 12 bands, the ladder contains vector dna fragments that. Generuler 1 kb plus dna ladder thermo fisher scientific.